Regular Intergeneric Hybridization of Leuciscine Cyprinids (Cyprinidae, Leuciscinae) in the Dnipro Affluants

Keywords: Cyprinidae, bream, roach, bleak, rudd, hybridization, allozymes, morphometry


The large-scale hybridization of fishes of the subfamily Leuciscinae in the subordinate systems of the Dnieper river basin is presented by data on two pair of species, Rutilus rutilus and Abramis brama, Alburnus alburnus and Scardinius erythrophthalmus. The hybridization and occurrence of F1 hybrids are confirmed with allozyme spectra and morphological characters on series of samples. The presence of single individuals sharply deviating in morphological characters among the bleak and common rudd hybrids suggests limited backcrossing with rudd. A complete morphometric analysis of the characteristics of bleak and rudd hybrids has been carried out. The paper discusses the issue of intergeneric hybridization in nature, which is a unique phenomenon characteristic only of some groups of cyprinids. The most likely reason is the overestimation of the taxonomic status of European cyprinids, which is confirmed by the insignificant level of intergeneric genetic divergence.


Abbott, R., Albado, D., Ansell, S., Bierne, S. N., Boughman, J., Brelsford, A., Buerkle, C. A., Buggs, R., Butlin, R. K., Dieckmann, U., Eroukhmanoff, F., Grill, A., Cahan, S. H., Hermansen, J. S., Hewitt, G., Hudson, A. G., Jiggins, C., Jones, J., Keller, B., Marczewski, T., Mallet, J., Martinez-Rodriguez, P., Möst, S. Mullen, M., Nichols, R., Nolte, A. W., Parisod, C., Pfennig, K., Rice, A. M., Ritchie, M. G., Seifert, B., Smadja, C. M., Stelkens, R., Szymura, J. M., Väinölä, R., Wolf, J. B. W., Zinner, D. 2013. Hybridization and speciation. Journal of Evolutionary Biology, 26 (2), 229-246.

Arnold, M. L. 1992. Natural hybridization as an evolutionary process. Annual Review of Ecology and Systematics, 23, 237-261.

Avise, J. C. 1977. Is evolution gradual or rectangular? Evidence from living fishes. Proceeding National Academy of Sciences, 74 (11), 5083-5087.

Barton, N. H. 2001. The role of hybridization in evolution. Molecular Ecology, 10 (3), 551-568.

Beling, D. O. 1928. On the characteristics of ichthyofauna of State Nature Reserve "Koncha-Zaspa". Zbirnyk Prats Derzhavnoho rybnoho zapovidnyka "Koncha-Zaspa", 1, 84-101 [In Ukrainian].

Berg, L.S. 1912. Fishes (Marsipobranchii and Pisces). Fauna of Russia and adjacent countries, 3 (1), St-Petersburg, 1-335 [In Russian].

Berinkey, L. 1960. Further Morphological and Osteological Investigations on the Hybrids of Hungarian Cyprinids. Annales Historico-Naturales Musei Nationalis Hungarici, 52, Pars Zoologica, 447-464.

Blachuta, J., Witkowski, A. 1984. Natural hybrids Alburnus alburnus (L.) × Rutilus rutilius (L.), Alburnus alburnus (L.) × Blicca bjoerkna (L.) and Alburnus alburnus (L.) × Abramis brama (L.) from the Oder River. Acta Hydrobiologica, 25-26 (2), 189-204.

Brennan, A. C., Woodward, G., Seehausen, O., Muñoz-Fuentes, V., Moritz, C., Guelmami, A., et al. 2015. Hybridization due to changing species distributions: adding problems or solutions to conservation of biodiversity during global change? Evolutionary Ecology Research. 16, 475-491.

Briolaya, J., Galtierb, N., Britoc, R.M., Bouvetd, Y. 1998. Molecular Phylogeny of Cyprinidae Inferred fromcytochrome bDNA Sequences. Molecular Phylogenetics and Evolution, 9 (1), 100-108.

Economidis, P. S., Wheeler, A. 1989. Hybrids of Abramis brama with Scardinius erythrophthalmus and Rutilus rutilus from Lake Volvi, Macedonia, Greece. Journal of Fish Biology, 35, 295-299.

Fahy, E., Martin, S., Mulrooney, M. 1988. Interaction of roach and bream in an Irish reservoir. Archives of Hydrobiology, 144, 291-309.

Golubtsov, A. S., Ilyin, I. I., Shaykin, A. V. 1990. Electroforetic identification of natural hybrids of the common bream Abramis brama with common roach Rutilus rutilus in Mozhayskoye water reservoir. Voprosy ikhtiologii, 30, 870-874 [In Russian].

Hänfling, B. 2000. Phylogenetics of European cyprinids: Insights from allozymes. Journal of Fish Biology, 57 (2), 265-276.

Hayden, B., McLoone, P., Coyne, J., Caffrey, J. M. 2014. Extensive hybridisation between roach, Rutilus rutilus L., and common bream, Abramis brama L., in Irish Lakes and Rivers. Biology and Environment: Proceedings of the Royal Irish Academy, 114B (1), 35-39.

Hubbs, C. L. 1955. Hybridization between fish species in nature. Systematic Zoology. 4 (1). 1-20.

Kammerad, B., Wuestemann, O. 1989. Occurrence of a hybrid between dace Leuciscus leuciscus and bleak Alburnus alburnus Teleostei Cyprinidae. Zoologischer Anzeiger, 222,170-176.

Kassambara, A., Mundt, F. 2017. Factoextra: extract and visualize the results of multivariate data analyses. R package version 1.0.5.

Kodukhova, Yu. V., Karabanov, D. P. 2013. Features of natural hyrids of common bream Abramis brama (Linnaeus, 1758) and common roach Rutilus rutilus (Linnaeus, 1758) in Rybinsk water reservoir. Izvestiia Samarskogo nauchnogo tsentra RAN, 15, 3 (7), 2293-2300 [In Russian].

Konopiński, M. K., Amirowicz A. 2018. Genetic composition of a population of natural common bream Abramis brama × roach Rutilus rutilus hybrids and their morphological characteristics in comparison with parent species. Journal of Fish Biology, 92 (2), 365-385.

Kottelat, M., Freyhof, J. 2007. Handbook of European freshwater fishes. Delemont. IUCN, 660.

Kuparinen, A., Vinni, M., Teacher, A. G. F., Kähkönen, K., Merilä, J. 2014. Mechanism of hybridization between bream Abramis brama and roach Rutilus rutilus in their native range. Journal of Fish Biology, 84 (1), 237-242.

Kovalchuk, O. M. 2017. Regional fish-based biostratigraphy of the Late Neogene and Pleistocene of southeastern Europe. Vestnik Zoologii, 51 (5), 375-392.

Le, S., Josse, J., Husson, F. 2008. FactoMineR: an R package for multivariate analysis. Journal of Statistical Software, 25 (1), 1-18.

Mayr, E. 1963. Animal species and evolution. Belknap Press of Harvard University Press, Cambridge, 1-797.

Mezhzherin, S. V. 1997. Punctualism or gradualism: data on genetic differentiation of small mammals of the Palaearctic. Genetika, 33 (4), 518-523 [In Russian].

Mezhzherin, S. V., Kokodiy, S. V., Kulish, A. V., Verlatii, D. B., Fedorenko, L. V. 2012. Hybridization of crucian carp (Carassius carassius (Linnaeus, 1758)) in Ukrainian reservoirs and genetic structure of hybrids. Cytology and genetics, 46 (1), 37-46 [In Russian].

Movchan, Yu. V. 2011. Ryby Ukrainy, Zoloti Vorota, Kyiv, 1-420 [In Ukrainian].

Movchan, Yu. V., Manilo, L. G., Smirnov, A. I., Scherbukha, A. Ya. 2003. Cyclostomata and fishes. Zoological Museum of the National Museum of Natural History of NAS of Ukraine, Kiev, 1-241 [In Russian].

Nelson, J. S., Grande, T. C., Wilson, M. V. H. 2016. Fishes of the World. 5th ed. Hoboken NJ, New York: John Wiley & Sons Inc., 1-752.

Nikolyukin, N. I. 1952. Interspecific hybridization of fishes. Saratov, Saratov, 312 [In Russian].

Peacock, A. G., Buntig, S. L. 1965. Serum protein electrophoresis in acrylamide gel patterns from normal human subjects. Science, 147, 1451-1453.

Pitts, C. S., Jordan, D. R., Cowx, I. G., Jones, N. V. 1997.Controlled breeding studies to verify the identity of roach and common bream hybrids from a natural population. Journal of Fish Biology, 51 (4), 686-696.

Perea, S., Böhme, M., Zupančič, P., Freyhof, J., Šanda, R., Özuluğ, M., Abdoli, A., Doadrio, I. 2010. Phylogenetic relationships and biogeographical patterns in Circum-Mediterranean subfamily Leuciscinae (Teleostei, Cyprinidae) inferred from both mitochondrial and nuclear data. BMC Evolutionary Biology, 10, 1-265.

Pervozvanskiy, V. Ya. Zelinskiy, Yu. P. 1981.Morphological and karyological features of hybrids of roach Rutilus rutilus (L.) and bream Abramis brama (L.) in lake Lososinnoye (lake Onego basin). Zoologicheskii zhurnal, 60 (3), 388-397 [In Russian].

Pravdin, I. F. 1966. Fish study manual. Pischevaya promyshlennost, Moscow, 376 [In Russian].

R Core Team. 2020. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.

Schwartz, F. J. 1972. World literature to fish hybrids, with an analysis by family, species, and hybrid. Publications of the Gulf Coast Research Laboratory Museum, 32, 1-328.

Scribner, T., Page, K. S., Bartron, M. L. 2000. Hybridization in freshwater fishes: review of case studies and cytonuclear methods of biological inference. Reviews in Fish Biology and Fisheries, 10 (3), 293-323.

Slynko, Yu. V. 2000.System of reproduction of intergeneric hybrids of roach (Rutilus rutilus L.), bream (Abramis brama L.) and blue bream (Abramis balerus L.) (Leuciscinae: Cyprinidae). Theses to obtain the scientific degree of Doctor of Philosophy, biological sciences, St.-Peterburg, 1-18 [In Russian].

Vasiliev, V. P. 1985. Evolutionary karyology of fishes. Nauka, Moscow, 1-299 [In Russian].

Velykokhatko, F. D. 1929. On the hybrid of Scardinius erythrophthalmus (L.) Х Alburnus alburnus (L.). Zapysky Nizhynskogo instytutu narodnoi osvity. Nizhyn, 9, 277-282 [In Ukrainian].

Vinyoles, D., Robalo, J. I., Sostoa, A., Almodóvar, A., Elvira, B., Nicola, G. G., Fernández-Degado, C., Santos, C. S., Doadrio, I., Sardá-Palomera, F., Almada, V. C. 2007. Spread of the alien bleak Alburnus alburnus (Linnaeus, 1758) (Actinopterygii, Cyprinidae) in the Iberian Peninsula: The role of reservoir. Graellsia, 63 (1), 101-110.

Wheeler, A. 1978. Hybrids of bleak, Alburnus alburnus, and chub, Leuciscus cephalus in English rivers. Journal of Fish Biology, 13 (4), 467-473.

Witkowski, A., Blachuta, J. 1980. Natural hybrids Alburnus alburnus (L.) × Leuciscus cephalus (L.) and Rutilus rutilus (L.) × Abramis brama (L.) from the rivers San and Biebza, Acta Hydrobiol, 22 (4), 473-487.

Wood, A. B., Jordan, D. R. 1987. Fertility of roach × bream hybrids. Rutilus rutilus (L.) × Abramis brama (L.) and their identification. Journal of Fish Biology, 30, 249-261.

How to Cite
Tsyba, A. A., Ghazali, M., Kokodiy, S. V., & Mezhzherin, S. V. (2021). Regular Intergeneric Hybridization of Leuciscine Cyprinids (Cyprinidae, Leuciscinae) in the Dnipro Affluants. Zoodiversity, 55(4). Retrieved from
Fauna and Systematics