Spatial Segregation of Diploid and Polyploids Spined Loaches (Сobitis elongatoides–taenia–tanaitica) in the River Systems of Western and Central Ukraine
Abstract
The investigation examines the structure of diploid and polyploid populations within the spined loach group (Сobitis elongatoides–taenia–tanaitica) in the water systems of Western and Central Ukraine, including the Danube River, Dniester River, Southern Bug River, Vistula River, Middle and Upper Dnipro River basins. The study reveals a bipolarity in settlement structure, with a sharp prevalence of either diploids or polyploids, and no discernible geographical trends in spatial distribution. Clonal hybrid polyploids, which make up 70 % of the individuals studied in the region, are most common in settlements along the main river channels, as well as in the middle and lower reaches of first and second-order tributaries. Populations where diploid specimens of the parent species are predominant are located in accessory systems, tributaries of the third and higher orders, upper reaches of first and second-order tributaries, and lakes. This spatial segregation arises from historical circumstances rather than ecological preferences, primarily due to the distance from water corridors through which polyploids invaded in the 1960s‒1970s. The displacement of diploid individuals by clonal hybrid polyploids is a universal phenomenon. This is due to the advantages of parthenogenetic reproduction and higher survival rates of polyploids in modern environmental situations.
References
Barton, N. H. & Hewitt, G. M. 1985. Analysis of hybrid zones. Annual review of ecology and systematic, 16, 113-148.
https://doi.org/10.1146/annurev.es.16.110185.000553
Bartoš, O., Röslein, J., Kotusz, J., Paces, J., Pekárik, L., Petrtýl, M., Halačka, K., Štefková, K. E., Mendel, J., Boroń, A., Juchno, D., Leska, A., Jablonska, O., Benes, V., Šídová, M., & Janko, K. 2019. The legacy of sexual ancestors in phenotypic variability, gene expression, and homoeolog regulation of asexual hybrids and polyploids. Molecular Biology and Evolution, 36, 1902-1920.
https://doi.org/10.1093/molbev/msz114
Bogart, J. P., Lowcock, L. A., Zeyl, C. W. & Mable, B. K. 1987. Genome constitution and reproductive biology of hybrid salamanders, genus Ambystoma, on Kelleys Island in Lake Erie. Canadian Journal of Zoology. 65 (9), 2188-2201.
https://doi.org/10.1139/z87-333
Bohlen, J. & Rab, P. 2001. Species and hybrid richness in spined loaches of the genus Cobitis (Teleostei: Cobitidae), with a cheklist of European forms and suggestions for conservation. Journal of Fish Biology, 59, 75-89.
https://doi.org/10.1006/jfbi.2001.1751
Boroń, A., 2003. Karyotypes and cytogenetic diversity of the genus Cobitis (Pisces: Cobitidae) in Poland: a review. Cytogenetic evidence for a hybrid origin of some Cobitis triploids. Folia Biologica, 51, 49-54.
Cunha, C., Doadrio, I., Abrantes, J. & Coelho, M. M. 2010. The evolutionary history of the allopolyploid Squalius alburnoides (Cyprinidae) complex in the northern Iberian Peninsula. Heredity, 106, 100-112.
https://doi.org/10.1038/hdy.2010.70
FishBase. 2017. Rainer, F. & Daniel, P. eds. Species of Cobitis in FishBase. June 2017 version. https://fishbase. mnhn.fr/identification/SpeciesList.php?genus=Cobitis.
Janko, K., Bohlen, J., Lamatsch, D., Flajahans, M., Epplen, J.T., Rab, P., Kotlik, P. & Alechtova, V. 2007 а. The gynogenetic reproduction of diploid and triploid hybrid spined loaches (Cobitis; Teleostei), and their ability to establish successful clonal lineages - on the evolution of polyploidy in asexual vertebrates. Genetica, 131, 185- 94.
https://doi.org/10.1007/s10709-006-9130-5
Janko, K., Flajshnans, M., Choleva, L., Bohlen J., ŠLechtová V., Rábová M., Lajbne, Z., Šlechta V., Ivanova, P., Dobrovolov, I., Culling M., Persa H., Kotusz J., & Ráb P. 2007 b. Diversity of European spined loaches (genus Cobitis, L.) an update of the geographic distribution of the Cobitis taenia hybrid complex with a description of new molecular tools for species and hybrid determination. Journal of Fish Biology, 71, 387-408.
https://doi.org/10.1111/j.1095-8649.2007.01663.x
Kim, I. S. & Lee, E. H. 1990. Diploid-triploid hybrid complex of the spined loach Cobitis sinensis and C. longicorpus (Pisces: Cobitidae). Korean Journal of Zoology. 1, 203-210.
Lusk, S., Koščo, J., Halačka, K., Luskova, V. & Flajšmans, M. 2003. Identification of Cobitis from Slovakian part of Tizsa basin. Folia Biologica, 51, Suppl., 61-65.
Luskova, V., Koščo, J., Halačka, K., Straňai, I., Lusk, S., Flajšhahs, M. 2004. Status of populations of the genus Cobitis in Slovakia. Biologia, 59 (5), 621-626.
Martins, M. J., Colares-Pereira, M. J., Cowx G. & Coelho, M. M. 1998. Diploids v. triploids of Rutilus alburnoides: spatial segregation and morphological differences. Journal of Fish Biology, 52, 817-828.
https://doi.org/10.1111/j.1095-8649.1998.tb00822.x
Mezhzherin, S. V. & Chudakorova, T. Yu., 2001. Expansion of triploid unisexual spined loaches Cobitis taenia L., 1758 (Cypriniformes, Cobitidae) in the watercourses of Ukraine. Reports of the National Academy of Sciences of Ukraine, 9, 153-157 [In Russian].
Mezhzherin, S. V. & Chudakorova T. Yu., 2002. Genetic structure of the diploid polyploid complex of spined loaches Cobitis taenia (Cypriniformes: Cobitidae) of the Middle Dnipro drainage. Russian Journal of Genetics, 38, 86-92.
Mezhzherin, S. V. & Pavlenko, L. I. 2009. Species composition and diversity of hybrid biotypes of the genus Cobitis (Cypriniformes, Cobitidae) of the fauna of Ukraine. Reports of the National Academy of Sciences of Ukraine, 2009, 10, 172-178 [In Russian].
Mezhzherin, S. V. & Pavlenko, L. I. 2010. Genetic diversity, origin, and geographic distribution tendencies of biotypes of polyploid spined loaches (Cypriniformes, Cobitidae, Cobitis) in Ukraine. Cytology and Genetics, 44, 317-327.
https://doi.org/10.3103/S0095452710050105
Mezhzherin, S. V., Pukhtaevych, P. P. & Kokodiy, S. V. 2020. The polyclonal structure of unisexual Prussian carp (Carassius gibelio (Bloch, 1782)) settlements in Northern Ukraine: comparison analysis of allozyme markers and chromosome numbers. Cytology and Genetics, 54, 55-62.
https://doi.org/10.3103/S0095452720010119
Mezhzherin, S. V., Kryvokhyzha, D., Tsyba, A. A. & Rostovskaya, O. V. 2022 a. Spined loache settlements structure (Cobitidae) of the Eastern Ukraine river systems and alternative character of dioloid and polyploidy populations // Zoodiversity, 56, 331-340.
https://doi.org/10.15407/zoo2022.04.331
Mezhzherin, S. V., Tsyba, A. A. & Kryvokhyzha, D. 2022 b. Cryptic expansion of hybrid polyploid spined loaches Cobitis in the rivers of Eastern Europe. Hydrobiologia, 849, 1689-1700.
https://doi.org/10.1007/s10750-022-04813-z
Moore, W. S., Rush, M. R. & Schultz, R. J. 1970. Distribution, adaptation and probable origin of an all-female form of Poeciliopsis (Pisces: Poecilidae) in NorthWestern Mexico. Evolution, 24, 789-795.
https://doi.org/10.1111/j.1558-5646.1970.tb01813.x
Peacock, F. C., Bunting, S. L. & Queen, K. G. 1965. Serum protein electrophoresis in acrylamide gel patterns from normal human subjects, Science. 147, 1451-1455.
https://doi.org/10.1126/science.147.3664.1451
Perdices, A., Bohlen, J. & Doadrio, I. 2008. The molecular diversity of adriatic spined loaches (Teleostei, Cobitidae). Molecular Phylogenetics and Evolution, 46, 382-390.
https://doi.org/10.1016/j.ympev.2007.05.007
Perdices, A., Bohlen, J., Slechtova, V. & Doadrio, I. 2016. Molecular evidence for multiple origins of the European spined loaches (Teleostei, Cobitidae). PLoS ONE, 11 (1): e0144628.
https://doi.org/10.1371/journal.pone.0144628
Rab, P., Rabova, M., Bonlen, J. & Lusk, S. 2000. Genetic differentiation of the two hybrid diploid-polyploid complexes of loaches, genus Cobitis (Cobitidae) involving C. taenia, C. elongatoides and C. sp. in the Czech Republic: кaryotype and cytogenetic diversity. Folia Zoologica, 49, 55-66.
Rasch, E. M. & Balsano, J. S. 1974. Biochemical and cytogenetic studies of Poecilia from Eastern Mexico II. Frequency, perpetuation and probable origin of triploid genomes in females associated with Poecilia formosa. International Journal of Tropical Biology and Conservation, 21, 351-381.
Shimuzu, Y., Oshiro, Ò. & Sakaizumi, M. 1993. Electrophoretic studies of diploid, triploid, and tetraploid forms of the Japanese silver crucian carp, Carassius auratus langsdorfii. Japan Journal of Ichthyology, 40, 65-75.
Šlechtova, V., Luskova, V., Slechta, V., Lusk, S., Halacka, K. & Bohlen, J. 2000. Genetic differentiation of two diploid-polyploid complexes of spined loach, genus Cobitis (Cobitidae), in the Czech Republic, involving C. taenia, C. elongatoides, and C. spp.: allozyme interpopulation and interspecific differences. Folia Zoologica. 49, 67-78.
Šlechtova, V., Luskova, V., Slechta, N. V., Lusk, S. & Pivonkova, J. 2003. Potential species identification by allozyme/protein markers in European spined loaches, Folia Biologica. 57, 43-47.
Sezaki, K, Watabe, S., Ochiai, Y. & Hashimoto, K. 1994. Biochemical genetic evidence for a hybrid origin of spined loach, Cobitis taenia taenia in Japan. Fish Biology. 44, 683-691.
https://doi.org/10.1111/j.1095-8649.1994.tb01244.x
Sezaki, K. & Kobayashi, H. 1978. Comparison of erythrocytic size between diploid and tetraploid in spinous loach C. biwae. Bulletin of the Japanese Society for the Science of Fish, 44, 851-864.
https://doi.org/10.2331/suisan.44.851
Ueno, K. & Ojima, Y. 1976. Diploid-tetraploid complexes in the genus Cobitis (Cobitidae, Cyprinida). Proceedings of the Japan Academy, 52, 446-449.
https://doi.org/10.2183/pjab1945.52.446
Vasil'ev, V. P. 1985. Evolutionary karyology of fish. Nauka, Moskow, 1-300 [In Russian].
Vasil'ev, V. P., Vasil'evа, К. D. & Osinov, A. G. 1989. Evolution of a diploid-triploid-tetraploid complex in fishes of the genus Cobitis (Pisces, Cobitidae). Evolution and ecology of unisexual vertebrates / Eds R. M. Dawley, J. P. Bogart. Albany; New York : New York State Museum, 466, 153-169.
Vasilʹev, V. P. & Vasilʹeva, K. D. 2022. Triploid forms' karyotypes of spined loaches from the genus Cobitis (Actinopterygii: Cypriniformes: Cobitidae) of the upper Dnipro and Western Dvina rivers: Analysis of the triploids' origin. Acta Ichthyologica et Piscatoria. 52, 67-75.
https://doi.org/10.3897/aiep.52.81191
Warren, W. C., García-Pérez, R., Xu, S. & Lampert, K. 2018. Clonal polymorphism and high heterozygosity in the celibate genome of the Amazon molly. Nature Ecology & Evolution, 2, 669-679.
