Integrative Approaches for Precise Identification and Range Delimitation of the Three Most Common European Leiopus species (Coleoptera, Cerambycidae)
Abstract
Leiopus nebulosus (Linnaeus, 1758), L. linnei Wallin, Nylander & Kvamme, 2009 and L. femoratus Fairmaire, 1859 are the three most common European species of the long-horn beetle genus Leiopus Audinet-Serville, 1835. These species have often been misidentified due to their general morphological similarities. This study addresses the need for a simple and reliable method to differentiate L. nebulosus, L. linnei, and L. femoratus. Through the examination of a long series of specimens, specific morphological traits were identified, such as the shape of the femora on all legs and the width of the prosternal process, which allow for rapid and accurate differentiation between the three species. The findings led to the refinement of the diagnostic criteria for these species and the development of a straightforward identification key. Ecological niche modelling was employed to construct accurate global distribution maps for all three species. Additionally, the proposal to conserve the name Leiopus linnei under Article 23.9 of the Code was made.
References
Alekseev, V. I. 2020. Interesting observations of beetles (Coleoptera) from Kaliningradskaya Oblast during 2018-2019, with supplementary pre-2018 data. Euroasian Entomological Journal, 19, 18-30.
https://doi.org/10.15298/euroasentj.19.1.02
Anisimova, M. & Gascuel, O. 2006. Approximate likelihood ratio test for branches: A fast, accurate and powerful alternative. Systematic Biology, 55 (4), 539-552.
https://doi.org/10.1080/10635150600755453
Babytskiy, A. I., Geryak, Yu. M., Zamoroka, A. M., Kavurka, V. V., Korneyev, V. O., Nazarenko, V. Yu., Popov, G. V., Prokhorov, A. V., Pushkar, T. I., Fursov, V. M. & Cherney, L. S. 2023. Materials for the fauna of invasive alien insects (Insecta) of Ukraine. Ukrainska entomofaunistyka, 14 (3), 1-29. (in Ukrainian)
Barteniev, O. F. 2009. The longhorn beetles in Left-coast Ukraine and Crimea. Proceeding V.N. Karazin Kharkiv National University, 1-418 [In Russian].
Bense, U. 1995. Longhorn Beetles: Illustrated Key to the Cerambycidae and Vesperidae of Europe. Weikersheim: Margraf, 1-512.
Biscaccianti, A. B. & Petruzziello, L. 2007. Leiopus femoratus in Central Apennine (Coleoptera Cerambycidae). Bollettino dell'Associazione romana di entomologia, 62 (1-4), 105-110.
Brustel, H., Berger, P. & Cocquempot, C. 2002. Catalogue des Vesperidae et des Cerambycidae de la faune de France (Coleoptera). Annales de la Société Entomologique de France, 38 (4), 443-461.
https://doi.org/10.1080/00379271.2002.10697354
Bukejs, A. & Balalaikins, M. 2011. New records of beetles (Insecta: Coleoptera) in Estonia. Acta Zoologica Lituanica, 21 (3), 235-237.
https://doi.org/10.2478/v10043-011-0022-2
Comelade, J. 2023. Notes de terrain: contribution à l'inventaire des coléoptères des Pyrénées-Orientales. Revue de l'Association Roussillonnaise d'Entomologie (R.A.R.E.), 32 (3), 178-182.
Danilevsky, M. L. 2020. Catalogue of Palaearctic Coleoptera. Volume 6/1. Chrysomeloidea I (Vesperidae, Disteniidae, Cerambycidae) Updated and Revised Second Edition. Leiden: Koninklijke Brill, 1-712.
https://doi.org/10.1163/9789004440333_002
Danilevsky, M. L. & Tavakilian, G. 2022. Additions and corrections to the Catalogue of Palaearctic Coleoptera, vol. 6/1, 2020. Revised and Updated Second Edition. Chrysomeloidea I (Vesperidae, Disteniidae, Cerambycidae). Part II. Humanity space International almanac, 11 (2), 107-171.
https://doi.org/10.15298/rusentj.30.2.09
Doychev, D., Topalov, P., Zaemdzhikova, G., Sakalian, V. & Georgiev, G. 2018. Additions to xylophagous longhorn beetles (Coleoptera: Cerambycidae) host plants in Bulgaria. Silva Balcanica, 19, 47-54.
Drumont, A. & Wallin, H. 2009. First record of Leiopus linnei Wallin, Nylander & Kvamme, 2009 in Belgium (Coleoptera, Cerambycidae, Lamiinae). Lambillionea, 109, 529.
Gutowski, J.M., Hilszczański, J., Kubisz, D., Kurzawa, J., Miłkowski, M., Mokrzycki, T., Plewa, R., Przewoźny, M. & Wełnicki, M. 2010. Distribution and host plants of Leiopus nebulosus (L.) and L. linnei Wallin, Nylander et Kvamme (Coleoptera: Cerambycidae) in Poland and neighbouring countries. Polish Journal of Entomology, 79, 271-282.
Dunskis, A. & Barševskis, A. 2018. Catalogue of longhorn beetles (Coleoptera: Cerambycidae) of Latvia. Acta Biologica Universitatis Daugavpiliensis, 18 (2), 165-198.
Gascuel, O. 1997. BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Molecular Biology Evolution, 14 (7), 685-695.
https://doi.org/10.1093/oxfordjournals.molbev.a025808
Górski, P. & Tatur-Dytkowski, J. 2015. Longhorn beetles (Coleoptera: Cerambycidae) of central Mazovia, Poland. Baltic Journal of Coleopterology, 15 (2), 107-127.
Gouy, M., Tannier, E., Comte, N. & Parsons, D.P. 2021. Seaview Version 5: A Multiplatform Software for Multiple Sequence Alignment, Molecular Phylogenetic Analyses, and Tree Reconciliation. Methods in Molecular Biology, 2231, 241-260.
https://doi.org/10.1007/978-1-0716-1036-7_15
Gradinarov, D. & Petrova, Y. 2019. Longhorn beetles (Coleoptera: Cerambycidae) from Vrachanska Planina Mountains and Vrachanski Balkan Nature Park. In: Bechev, D. & Georgiev, D., eds. Faunistic Diversity of Vrachanski Balkan Nature Park. Part 2, Zoonotes Supplement. Plovdiv University Press, 7, 59-80.
Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59 (3), 307-321.
https://doi.org/10.1093/sysbio/syq010
Hedyessy, G. & Kutasi, Cs. 2010. First record of Leiopus femoratus Fairmaire, 1859 in Hungary (Coleoptera: Cerambycidae). Folia entomologica Hungarica, 71, 43−45.
Hendrich, L., Moriniere, J., Haszprunar, G., Hebert, P., Hausmann, A., Köhler, F. & Balke, M. 2014. A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3500 identified species to BOLD. Molecular Ecology Resources, 15 (4), 795-818.
https://doi.org/10.1111/1755-0998.12354
Klausnitzer, B., Klausnitzer, U., Wachmann, E. & Hromádko, Z. 2016. Die Bockkäfer Mitteleuropas. Cerambycidae. Band 2: Die mitteleuropäischen Arten. Magdeburg: Verlags KG Wolf: 3-303.
Kvamme, T. & Wallin, H. 2013. Biological notes and distribution of Leiopus Audinet-Serville, 1835 (Coleoptera, Cerambycidae) in Norway. Norwegian Journal of Entomology, 60 (1), 119-125.
Lacoste, F. 2013. Un nouveau venu pour le département du Puy-de-Dôme (F-63) Leiopus linnei Wallin, Nýlander & Kvamme, 2009 (Coleoptera, Cerambycidae). Le Coléoptériste, Bulletin de liaison de l'ACOREP, 16 (3), 1-143.
Löbl, I. & Smetana, A. 2010. Catalogue of Palaearctic Coleoptera. Volume 6 Chrysomeloidea I. Stenstrup: Apollo Books, 1-924.
https://doi.org/10.1163/9789004260917_001
Merkl, O. 2008. Data to the knowledge on the beetle fauna of Maramureş, Romania (Coleoptera). Studia Universitatis "Vasile Goldis", Seria Stiintele Vietii (Life Sciences Series), 18, 243-311 (supplement).
Micas, L. & Van Meer, C. 2020. Quelques données originales de Cerambycidae du Sud de la France (Coleoptera Cerambycidae). L'Entomologiste, 76 (3), 137-142.
Miłkowski, M. & Mokrzycki, T. 2021. Koleopterofauna czeremchy amerykańskiej Prunus serotina Ehrh. Wiadomości Entomologiczne, 40 (1), 40-50.
Monnerat, C., Chittaro, Y., Sanchez, A. & Gonseth, Y. 2015. Liste commentée des Lucanidae, Cetoniidae, Buprestidae et Cerambycidae (Coleoptera) de Suisse. Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 88 (1-2), 173-228.
Olbrycht, T. & Trzeciak, A. 2018. Kłokoczka południowa Staphylea pinnata L. jako roślina żywicielska chrząszczy kózkowatych (Coleoptera, Cerambycidae). Roczniki Bieszczadzkie, 26, 137-144.
Özdikmen, H. & Laz, B. 2022. Updated feeding preferences of Leiopus Audinet-Serville species in Turkey with new host plants for endemic longicorn beetle Leiopus syriacus abieticola Sama & Rapuzzi (Coleoptera: Cerambycidae: Lamiinae). Munis Entomology & Zoology, 17 (1), 301-315.
Plewa, R., Hilszczański, J. & Jaworski, T. 2014. Kózkowate (Coleoptera: Cerambycidae) z kolekcji dr. Bolesława Burakowskiego zebrane podczas wyprawy entomologicznej do Rumunii. Wiadomości Entomologiczne, 33 (2), 126-138.
Plewa, R., Marczak, D., Borowski, J., Mokrzycki, T., Jakubowski, M. & Górski, P. 2015. New data on the occurrence of longhorn beetles (Coleoptera: Cerambycidae) in the Republic of Macedonia. Acta Zoologica Bulgarica, 67 (1), 43-50.
Roques, A., Ren, L., Rassati, D., Shi, J., Akulov, E., Audsley, N., Auger-Rozenberg, M.-A., Avtzis, D., Battisti, A., Bellanger, R., Bernard, A., Bernadinelli, I., Branco, M., Cavaletto, G., Cocquempot, C., Contarini, M., Courtial, B., Courtin, C., Denux, O., Dvořák, M., Fan, J.-T., Feddern, N., Francese, J., Franzen, E. K. L., Garcia, A., Georgiev, G., Georgieva, M., Giarruzzo, F., Gossner, M., Gross, L., Guarneri, D., Hoch, G., Hölling, D., Jonsell, M., Kirichenko, N., Loomans, A., Luo, Y.-Q., McCullough, D., Maddox, C., Magnoux, E., Marchioro, M., Martinek, P., Mas, H., Mériguet, B., Pan, Y.-Z., Phélut, R., Pineau, P., Ray, A. M., Roques, O., Ruiz, M.-C., Sarto i Monteys, V., Speranza, S., Sun, J.-H., Sweeney, J. D., Touroult, J., Valladares, L., Veillat, L., Yuan, Y., Zalucki, M. P., Zou, Y. & Žunič-Kosi, A. 2023. Worldwide tests of generic attractants, a promising tool for early detection of non-native cerambycid species. in Jactel, H., Orazio, C., Robinet, C., Douma, J. C., Santini, A., Battisti, A., Branco, M. & Seehausen, L. (eds.). 2023. Conceptual and technical innovations to better manage invasions of alien pests and pathogens in forests. NeoBiota, 84, 169-209.
https://doi.org/10.3897/neobiota.84.91096.figure1
Rossa, R., Goczał, J. & Tofilski, A. 2017. Hind wing morphology facilitates discrimination between two sibling species: Leiopus nebulosus and L. linnei (Coleoptera: Cerambycidae). Zootaxa, 4227 (2), 266-278.
https://doi.org/10.11646/zootaxa.4227.2.7
Ruchin, A.B., Egorov, L.V. & Khapugin, A.A. 2021. Usage of fermental traps for the study of the species diversity of Coleoptera. Insects, 12 (5), 407.
https://doi.org/10.3390/insects12050407
Sama, G. 2002. Atlas of the Cerambycidae of Europe and the Mediterranean Area. Volume 1: Northern, Western, Central and Eastern Europe British Isles and Continental Europe from France (excl. Cosrica) to Scandinavia and Urals. Zlín: Vít Kabourek, 1-173.
Siering, G. & Shumka, S. 2015. Die Bockkäfer-Fauna (Coleoptera, Cerambycidae) des Shkumbin-Tales und weiterer Gebiete bei Librazhd (Albanien). Entomologische Blätter für Biologie und Systematik der Käfer, 111, 459-463.
Tamutis, V., Tamutė, B. & Ferenca, R. 2011. A catalogue of Lithuanian beetles (Insecta: Coleoptera). ZooKeys, 121, 1-494.
https://doi.org/10.3897/zookeys.121.732
Touroult, J., Cima, V., Bouyon, H., Hanot, C., Horellou, A. & Brustel, H. 2019. Longicornes de France - Atlas préliminaire (Coleoptera: Cerambycidae & Vesperidae). Supplément au bulletin d'ACOREP-France, Paris, 1-176.
Troukens, W., Drumont, A., Raemdonck, H., Dekuijper, C. & Dahan, L. 2017. Nieuwe en interessante vondsten van boktorren (Coleoptera: Cerambycidae) in de omgeving van Brussel. Phegea, 45 (1), 13-18.
Vitali, F. 2018. Atlas of the Insects of the Grand-Duchy of Luxembourg: Coleoptera, Cerambycidae. Luxembourg: Musée national d'histoire naturelle, Ferrantia, 79, 1-208.
Wallin, H., Nylander, U. & Kvamme, T. 2009. Two sibling species of Leiopus Audinet-Serville, 1835 (Coleoptera: Cerambycidae) from Europe: L. nebulosus (Linnaeus, 1758) and L. linnei sp. nov. Zootaxa, 2010, 31-45.
https://doi.org/10.11646/zootaxa.2010.1.3
Zahajkevych, I. K. 1991. Taxonomy and ecology of Cerambycidae. Naukova Dumka, Kyiv, 1-420 [In Russian].
Zamoroka, A. M., Panin, R. Yu., Kapelukh, Ya. I. & Podobivskiy, S. S. 2012. The catalogue of the longhorn beetles (Coleoptera: Cerambycidae) of western Podillya, Ukraine. Munis Entomology & Zoology, 7 (2), 1145-1177.
Zamoroka, A. M. & Kapelyukh, Ya. I. 2012. The genus Leiopus Audinet-Serville, 1835 in Western Ukraine and the invading of Mediterranean-Pontic species Leiopus femoratus Fairmaire 1859 (Coleoptera: Cerambycidae: Acanthocinini). Scientific Bulletin of the Uzhhorod University. Series Biology, 32, 60-64.
Zamoroka, A. M. & Kapelyukh, Ya. I. 2016. Notes on the longhorn beetles (Coleoptera: Cerambycidae) of Nature Reserve "Medobory" (Ukraine). Ukrainska Entomofaunistyka, 7(4), 50-76.
Zamoroka, A. M. 2018. The longhorn beetles (Coleoptera: Cerambycidae) of the Eastern Carpathian Mountains in Ukraine. Munis Entomology & Zoology, 13 (2), 655-691.
Zamoroka, A. M., Semaniuk, D. V., Shparyk, V. Yu., Mykytyn, T. V. & Skrypnyk, S. V. 2019. Taxonomic position of Anastrangalia reyi and A. sequensi (Coleoptera, Cerambycidae) based on molecular and morphological data. Vestnik Zoologii, 53 (3), 209-226.
https://doi.org/10.2478/vzoo-2019-0021
Zamoroka, A. M. 2022 a, Molecular revision of Rhagiini sensu lato (Coleoptera, Cerambycidae): Paraphyly, intricate evolution and novel taxonomy. Biosystem Diversity, 30 (3), 295-309.
https://doi.org/10.15421/012232
Zamoroka, A. M. 2022 b. The longhorn beetles (Coleoptera, Cerambycidae) of Ukraine: results of two centuries of research. Biosystem Diversity, 30 (1), 46-74.
https://doi.org/10.15421/012206
Zamoroka, A. M., Trócoli, S., Shparyk, V. Yu. & Semaniuk, D. V. 2022. Polyphyly of the genus Stenurella (Coleoptera, Cerambycidae): Consensus of morphological and molecular data. Biosystem Diversity, 30 (2), 119-136.
https://doi.org/10.15421/012212
Zamoroka, A. 2023. New additions to the fauna of the longhorn beetles in Ukraine with a new record of rare, poorly known and invasive species. Baltic Journal of Coleopterology, 23 (2), 159-188.
Zamoroka, A. M. & Mykhailiuk-Zamoroka, O. V. 2023. Proposals for unification of the national nomenclature of longhorn beetles (Coleoptera: Cerambycidae) of the Ukrainian fauna and some exotic taxa. Part ІV: subfamily Lamiinae. Geo&Bio, 25, 141-156. [In Ukrainian].
https://doi.org/10.53452/gb2511
Zamoroka, A. M., Shparyk, V. Yu., Dovhanyuk, I. Ya. & Varga, O. O. 2023. Insects associated with the European mistletoe (Viscum album) in Western Ukraine: A pilot study. Zoodiversity, 57 (5), 433-450.
https://doi.org/10.15407/zoo2023.05.433
Zamoroka, A. & Zinenko, O. 2024. Taxonomic position of Tetrops peterkai Skořepa, 2020 (Coleoptera: Cerambycidae) and its cryptic distribution. Journal of Vasyl Stefanyk Precarpathian National University. Biology, 11, 6-19.