Species Characteristics of Anthropogenic Pollution Effect in the Pelophylax esculentus Water Frog Complex (Anura, Ranidae). Changes in the Histostructure of the Liver
Abstract
The paper examined the specific features of hepatocyte damage, parenchymal repair processes and pigment accumulation in the livers of water frogs (Pelophylax esculentus complex) from a moderately polluted agrocenosis. It was demonstrated that hybrid individuals exhibit significant differences from their parental species with regard to the peculiarities of pigment metabolism in the liver. All hybrid frogs have a low pigment content in their livers. Additionally, the hybrid frog sample has a significantly higher proportion of animals with degraded connective tissue. The parental species do not differ in these parameters. The features of genetic material transfer that cause hybrids to differ reliably from parental species in terms of the presence of histological changes in the liver are discussed.
References
Akulenko, N. M. 2012. Haemopoietic system of the anurans: the role of bone marrow and liver. Vestnik Zoologii, 46 (4), 347-354.
https://doi.org/10.2478/v10058-012-0027-8
Akulenko, N. M. 2015. Changes in liver parenchyma of green frogs (Pelophylax esculentus complex) under conditions of anthropogenic pollution and their use in monitoring water bodies. Vestnik Zoologii, 49 (5), 453-458.
https://doi.org/10.1515/vzoo-2015-0053
Akulenko, N. M. 2016. Peculiar features of hematopoiesis in the liver of mature and immature representatives of green frogs (Pelophylax esculentus complex). Vestnik Zoologii, 50 (6), 547-552.
https://doi.org/10.1515/vzoo-2016-0062
Akulenko, N. M. 2021. Liver pigment cells of the green frog (Pelophylax esculentus complex) are involved in hematopoiesis and liver parenchyma repair. International Journal of Scientific Research, 10 (10), 83-84.
https://doi.org/10.36106/ijsr/8731395
Akulenko, N. M., Zhalai E. I. & Nekrasova O. D. 2012 Hybrid complex of green frogs-an accident, evolutionary reserve, or adaptation process with limited opportunities? Proceedings of the international conference "XXVI Liubishchevskiye Readings. Modern Problems of Evolution" (Russian Federation, Ulyanovsk, April 5-7, 2012), Ulyanovsk State Pedagogical University, 61-67 [In Russian]
Bernabò, I, Guardia, A., Macirella, R., Tripepi, S. & Brunelli, E. 2017. Chronic exposures to fungicide pyrimethanil: multi-organ effects on Italian tree frog (Hyla intermedia). Scientific Reports, 7, 6869.
https://doi.org/10.1038/s41598-017-07367-6
Bove, P., Milazzo, P. & Barbuti, R. 2014. The role of deleterious mutations in the stability of hybridogenetic water frog complexes. BMC Evolutionary Biology, 14, 107.
https://doi.org/10.1186/1471-2148-14-107
Bucci, S., Ragghianti, M., Guerrini, F., Cerrini, V., Mancino, G., Morosi, A., Mossone, M. & Pascolini, R. 2000. Negative environmental factors and biodiversity: The case of the hybridogenetic green frog system from Lake Trasimeno. Italian Journal of Zoology, 67 (4), 365-370.
https://doi.org/10.1080/11250000009356341
Christiansen, D. G., Fog, K., Pedersen, B. V. & Boomsma, J. J. 2005 Reproduction and hybrid load in all-hybrid populations of Rana esculenta water frogs in Denmark. Evolution, Jun, 59 (6),1348-1361.
https://doi.org/10.1111/j.0014-3820.2005.tb01784.x
Di Rosa, I. , Simoncelli, F. , Fagotti, A. & Pascolini, R. 2006. The proximate cause of frog declines? Nature, 439, 161-167.
Dubey, S, Maddalena, T, Bonny, L, Jeffries, D. L., Dufresnes, Ch. 2019. Population genomics of an exceptional hybridogenetic system of Pelophylax water frogs. BMC Evol. Biol. 2019, 19: 164. Published online Aug 5.
https://doi.org/10.1186/s12862-019-1482-4
Fasola, E., Ribeiro, R. & Lopes, I. 2015, Microevolution due to pollution in amphibians: A review on the genetic erosion hypothesis. Environmental Pollution, 204, 181-190. .
https://doi.org/10.1016/j.envpol.2015.04.027
Gutierre, R. C., Jared, C., Antoniazzi, M. M., Coppi, A. A. & Egami, M. I. 2018. Melanomacrophage functions in the liver of the caecilian Siphonops annulatus. J Anat. Mar, 232 (3), 497-508.
https://doi.org/10.1111/joa.12757
Mezhzherin, S., Morozov-Leonov, S., Nekrasova, O. & Rostovskaya, O. 2023. Geographic peculiarities of structure and hemicloning reproduction of Pelophylax esculentus water frog complex (Anura, Ranidae) populations in the East European Plain within Ukraine. Amphibia-Reptilia, 44 (3), 263-275.
https://doi.org/10.1163/15685381-bja10136
Mikulíček, P., Kautman, M., Demovič, B. & Janko, K. 2014. When a clonal genome finds its way back to a sexual species: evidence from ongoing but rare introgression in the hybridogenetic water frog complex. Journal of Evolutionary Biology, 27 (3), 628- 642.
https://doi.org/10.1111/jeb.12332
Nekrasova, O. D. 2002. Interspecific variability and colouring polymorphism of green frogs Rana esculenta complex (Amphibia, Ranidae) in hybrid populations. Vestnik Zoologii, 36 (4), 47-54 (In Russian).
Nekrasova, O. D., Mezhzherin. S. V. & Morozov-Leonov, S. Yu. 2004. Demographic Structure of Rana esculenta (Amphibia, Ranidae) Complex Hybrid Populations from the Middle Dnieper Basin.Vestnik Zoologii, 38 (6), 47-56.
Pascolini, R., Daszak, P., Cunningham, A. A., Tei, S., Vagnetti, D., Bucci, S., Fagotti, A., Di Rosa, I. 2003. Parasitism by Dermocystidium ranae in a population of Rana esculenta complex in Central Italy and description of Amphibiocystidium n. gen. Diseases of aquatic organisms. Aug 15, 56 (1), 65-74.
https://doi.org/10.3354/dao056065
Plenet, S., Joly, P., Hervant, F., Fromont, E. & Grolet, O. 2005. Are hybridogenetic complexes structured by habitat in water frogs? Journal of Evolutionary Biology, 18, 1575-1586.
https://doi.org/10.1111/j.1420-9101.2005.00961.x
Prokić, M. D., Borković-Mitić, Sl. S., Krizmanić, I. I, Mutić, J. J , Trifković, J. Đ., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R, Radovanović, T. B, Pavlović, Sl. Z. & Saičić, Z. S. 2016 Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex. Ecotoxicology Oct; 25 (8), 1531-1542.
https://doi.org/10.1007/s10646-016-1707-x
Quilodrán, Cl. S., Montoya-Burgos, J. I. & Currat, M. 2015. Modelling interspecific hybridization with genome exclusion to identify conservation actions: the case of native and invasive Pelophylax waterfrogs. Evolutionary Applications. Feb; 8 (2), 199-210.
https://doi.org/10.1111/eva.12245
Shabanov, D. A., Zinenko, A. I., Korshunov, A. V., Kravchenko, M. A. & Mazepa, G. A. 2006. Study of population systems of green frogs (Rana esculenta complex) in Kharkiv region: history, current state, prospects. Vísnik Kharkívs'kogo natsíonal'nogo Uníversitetu. Ser. Bíologíya, 729 (3), 208-220.
