Scanning Electron Microscopy Provides a Novel Method to Map Abdominal Musculature in Archaeognatha (Insecta)
Abstract
Using scanning electron microscopy, previously overlooked cuticle modifications were revealed in the attachment points of skeletal musculature of Trigoniophthalmus simplex (Insecta, Archaeognatha, Machilidae). These findings demonstrate that SEM may serve as an indirect approach for rapidly and precisely mapping complicated abdominal musculature of Archaeognatha. The reference to Archaeognatha is especially relevant for the mapping of the abdominal muscles in insects in the sense of the presence of a hypothesized plesiomorphic sclerite composition in this group of wingless insects.
References
Bitsch, J. 1973. Morphologie abdominale des machilides (Insecta Thysanura) - I. Squelette et musculature des segments prégénitaux. Annales des Sciences Naturelles, Zoologie, Ser. 12, 15, 173-200.
Bitsch, J. 1974. Morphologie abdominale des machilides (Thysanura) - II. Squelette et musculature des segments génitaux femelles. International Journal of Insect Morphology and Embryology, 3 (1), 101-120.
https://doi.org/10.1016/S0020-7322(74)81010-X
Bitsch, C. & Bitsch, J. 2002. The endoskeletal structures in arthropods: cytology, morphology and evolution. Arthropod Structure & Development, 30 (3), 159-177.
https://doi.org/10.1016/S1467-8039(01)00032-9
Caveney, S. 1969. Muscle attachment related to cuticle architecture in Apterygota. Journal of Cell Science, 4 (2), 541-559.
https://doi.org/10.1242/jcs.4.2.541
Klass, K. D. & Matushkina, N. A. 2012. The exoskeleton of the female genitalic region in Petrobiellus takunagae (Insecta: Archaeognatha) : Insect-wide terminology, homologies, and functional interpretations. Arthropod Structure & Development, 41 (6), 575-591.
https://doi.org/10.1016/j.asd.2012.06.003
Klass, K. D. & Matushkina, N. A. 2018. The exoskeleton of the male genitalic region in Archaeognatha, with hypotheses on the early evolution and the morphological interpretation of genitalia in insects. Arthropod Systematics & Phylogeny, 76 (2), 235-294.
https://doi.org/10.3897/asp.76.e31928
Klass, K. D., Matushkina, N. A. & Kaidel, J. 2012. The gonangulum: a reassessment of its morphology, homology, and phylogenetic significance. Arthropod Structure & Development, 41 (4), 373-394.
https://doi.org/10.1016/j.asd.2012.03.001
Klug, R. & Klass, K. D. 2007. The potential value of the mid-abdominal musculature and nervous system in the reconstruction of interordinal relationships in lower Neoptera. Arthropod Systematics & Phylogeny, 65 (1), 73-100.
https://doi.org/10.3897/asp.65.e31666
Lieberman, Z. E., Billen, J., van de Kamp, T. & Boudinot, B. E. 2022. The ant abdomen: The skeletomuscular and soft tissue anatomy of Amblyopone australis workers (Hymenoptera: Formicidae). Journal of Morphology, 283 (6), 693-770.
https://doi.org/10.1002/jmor.21471
Matushkina, N. A. & Klass, K.-D. 2020. Male genitalia of Charimachilis (Insecta: Archaeognatha) and the status of archaeognathan "paleoforms". Organisms Diversity and Evolution, 20 (2), 253-266.
https://doi.org/10.1007/s13127-020-00439-y
Solis, M. A. & Metz, M. A. 2011. Male genital homology based on musculature originating from the tegumen in snout moths (Lepidoptera: Pyraloidea). Arthropod Structure & Development, 40 (5), 435-448.
https://doi.org/10.1016/j.asd.2011.03.005
Song, N., Li, X., Yin, X., Li, X., Yin, J. & Pan, P. 2019. The mitochondrial genomes of palaeopteran insects and insights into the early insect relationships. Scientific Reports, 9 (1), 1-11.
https://doi.org/10.1038/s41598-019-54391-9
Sturm, H. & Machida, R., 2001. Archaeognatha. In: Handbook of Zoology, vol. 4 (37). Walter de Gruyter, Berlin, 1-213.




